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Record W3133549674 · doi:10.1101/2021.03.09.434627

SSR1 is a vital regulator in plant mitochondrial iron-sulfur biosynthesis

2021· preprint· en· W3133549674 on OpenAlexafffund
Xuanjun Feng, Huiling Han, Jie Liu, Wenhan Ying, Yuanyuan Cai, Min Zhang, Yanli Lu, Rongmin Zhao, Xuejun Hua

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2021
Typepreprint
Languageen
FieldEnergy
TopicMetalloenzymes and iron-sulfur proteins
Canadian institutionsUniversity of Toronto
FundersInstitute of Botany, Chinese Academy of SciencesZhejiang Sci-Tech UniversityChinese Academy of SciencesNatural Sciences and Engineering Research Council of CanadaNational Natural Science Foundation of China
KeywordsMitochondrionBiosynthesisMutantBiologyBiochemistryRegulatorArabidopsisChaperone (clinical)Function (biology)ATP synthaseCell biologyGeneSulfur metabolismArabidopsis thalianaSulfurChemistry

Abstract

fetched live from OpenAlex

Abstract The Arabidopsis SHORT AND SWOLLEN ROOT1 ( SSR1 ) gene encodes a mitochondrial TPR domain-containing protein and was previously reported to function in maintaining mitochondria function. In a screen for suppressors of the short-root phenotype of the loss-of-function mutant ssr1-2 , two mutations, sus1 and sus2 (suppressor of ssr1-2 ), were isolated. sus1 and sus2 result from G87D and T55M single amino acid substitution in HSCA2 (At5g09590) and ISU1 (At4g22220), both of which are core components in iron-sulfur cluster biosynthesis pathway in mitochondria (ISC). We here demonstrated that SSR1 displayed a strong chaperone-like activity and was able to enhance the binding of HSCA2 to ISU1, an essential step for the normal operation of ISC machinery. Accordingly, the enzymatic activities of several iron-sulfur proteins, the mitochondrial membrane potential and ATP content are reduced in ssr1-2 . Interestingly, SSR1 appears to exist only in plant lineages, possibly conferring adaptive advantages on plant ISC machinery to environment.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.005

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.013
GPT teacher head0.201
Teacher spread0.188 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations2
Published2021
Admission routes2
Has abstractyes

Explore more

Same venuebioRxiv (Cold Spring Harbor Laboratory)Same topicMetalloenzymes and iron-sulfur proteinsFrench-language works237,207