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Record W3135060487 · doi:10.1073/pnas.2021157118

Cryo-EM structure and kinetics reveal electron transfer by 2D diffusion of cytochrome <i>c</i> in the yeast III-IV respiratory supercomplex

2021· article· en· W3135060487 on OpenAlexafffund
Agnes Moe, Justin M. Di Trani, John L. Rubinstein, Peter Brzezinski

Bibliographic record

VenueProceedings of the National Academy of Sciences · 2021
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicPhotosynthetic Processes and Mechanisms
Canadian institutionsUniversity of TorontoHospital for Sick Children
FundersCanadian Institutes of Health ResearchVetenskapsrådetNational Institute of General Medical SciencesKnut och Alice Wallenbergs StiftelseGovernment of Canada
KeywordsKineticsElectron transferDiffusionYeastElectron transport chainChemistryPhysicsBiochemistryThermodynamicsPhotochemistry

Abstract

fetched live from OpenAlex

Significance In the last steps of food oxidation in living organisms, electrons are transferred to oxygen through the membrane-bound respiratory chain. This electron transfer is mediated by mobile carriers, such as membrane-bound quinone and water-soluble cytochrome c . The latter transfers electrons from respiratory complex III to complex IV. In yeast, these complexes assemble into III 2 IV 1/2 supercomplexes, but its role has remained enigmatic. This study establishes a functional role for this supramolecular assembly in the mitochondrial membrane. We used cryo-EM and kinetic studies to show that cytochrome c shuttles electrons by two-dimensional diffusion, sliding along the surface of III 2 IV 1/2 . The structural arrangement of III 2 IV 1/2 supercomplexes suggests a mechanism to regulate cellular respiration.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.012
Threshold uncertainty score0.199

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.015
GPT teacher head0.263
Teacher spread0.248 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations66
Published2021
Admission routes2
Has abstractyes

Explore more

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