Study on the Auto-assembly of Truncated Phytochrome from Synechocystis sp.PCC7120
Bibliographic record
Abstract
Five truncated fragments of aphA gene were amplified from the genomic DNA of Synechocystis sp.PCC7120 by PCR,including aphA(26-320),aphA(27-320),aphA(28-320),aphA(29-320)and aphA(32-320).After expression via the vector pET30,reconstitutions of the expressed mutated apo-proteins with phycocyanobilin(PCB) were studied in vitro under certain conditions.The results showed:The chromoprotein that obtained from the reconstitution of AphA(26-320) had similar photochemical properties of plant phytochrome.The covalent binding of PCB with the chromoprotein was verified further by acid-urea denaturation and UV-induced fluorescence by Zn~(2+)-SDS gel tests.The maximal Pr/Pfr absorption of AphA(26-320) was at 660/610 nm.The other four truncated fragments,AphA(27-320),AphA(28-320),AphA(29-320),AphA(32-320) could not auto-assembly with PCB in the similar conditions,as no reversible photochemistry was detected.The results implicates that the lyase domain of AphA existing in AphA(26-320).
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".