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Record W3139854754 · doi:10.48336/4jfn-6s47

sRNARFTarget: a machine learning-based approach for sRNA target prediction

2022· dissertation· en· W3139854754 on OpenAlexaff
Kratika Naskulwar

Bibliographic record

VenueMemorial University Research Repository (Memorial University) · 2022
Typedissertation
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenomics and Phylogenetic Studies
Canadian institutionsMemorial University of Newfoundland
Fundersnot available
KeywordsTransfer RNAComputational biologyBiologyGenomicsGeneTranslation (biology)Gene expressionGeneticsGenomeRNAMessenger RNA

Abstract

fetched live from OpenAlex

Bacterial small regulatory RNAs (sRNAs) play a vital role in the regulation of gene expression in bacteria. sRNAs regulate gene expression by interacting with mRNAs or proteins. Bacterial sRNAs are involved in various processes, such as environmental stress response, metabolism, and virulence. We need to identify the mRNAs and/or proteins that these sRNAs interact with, to understand the functional roles of sRNAs. These mRNAs or proteins are called targets of the sRNAs. There are several computational tools available for sRNA target prediction; however, these tools have a high number of false positives, and the most accurate tool requires sRNA sequence conservation across bacteria. As a result of this research project, a machine-learning-based method (sRNARFTarget) for sRNA target prediction applicable to any bacterium or sRNA has been developed. In this thesis, we show that sRNARFTarget substantially outperforms current non-comparative genomics-based methods in terms of running time and ranking of true interactions.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.002
metaresearch head score (Gemma)0.003
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Simulation or modeling · Consensus signal: none
GenreCandidate signal: Methods · Consensus signal: Methods
Teacher disagreement score0.004
Threshold uncertainty score0.013

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0020.003
Meta-epidemiology (narrow)0.0020.001
Meta-epidemiology (broad)0.0010.002
Bibliometrics0.0020.001
Science and technology studies0.0010.000
Scholarly communication0.0010.001
Open science0.0020.001
Research integrity0.0010.002
Insufficient payload (model declined to judge)0.0040.002

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.018
GPT teacher head0.245
Teacher spread0.228 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designSimulation or modeling
Domainnot available
GenreMethods

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2022
Admission routes1
Has abstractyes

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