Construction and Application of Subgenomic Replicon Vectors of Japanese Encephalitis Virus
Bibliographic record
Abstract
Based on the infectious clone of JEV vaccine SA14-14-2,the subgenomic replicons pCTCJEV,pCTMJEV with large deletions in the structural region were constructed.Then they were transfected into BHK21 cell,the RNA replication of JEV subgenome can be monitored by RT-PCR and the non-structural protein can be found expressed in the cell by IFA.To explore the possibility of using a reporter gene assay to monitor synthesis of the positivestrand and the negative-strand JEV RNA,we inserted an enhanced green fluorescence protein(EGFP) gene into the 3′-UTR of pCTCJEV,pCTMJEV under the control of the internal ribosomal entry site(IRES) of encephalomyelocarditis virus RNA.After transfection,the EGFP fluorescence could be seen under the fluorescence microscope 1 day later,and maintained for more than a week with no apparent cytopathic effect.The constructed JEV replicons would provide valuable tools to provide a possible vector for a long-lasting RNA virus expression system.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".