葡萄糖转运的胰岛素敏感性以及forskolin, dipyridamole和pentobarbital对三种L6 <BR>骨骼肌细胞葡萄糖转运的抑制作用 <BR>
Bibliographic record
Abstract
用稳定过表达并带有myc表位的葡萄糖转运子1(glucose transporter 1, GLUT1)或葡萄糖转运子4(glucose transporter 4, GLUT4)的L6骨骼肌细胞株定征GLUT1和GLUT4对胰岛素的响应. 所筛选的L6-GLUT1myc细胞克隆分化前后的葡萄糖摄取量均在线性范围. 100 nmol/L胰岛素使L6-GLUT1myc和L6-GLUT4myc肌原细胞膜上GLUT1或GLUT4的量分别达到基础组的(1.58±0.01)倍和(1.96±0.11)倍, 2-脱氧葡萄糖摄取量分别达到了(1.53±0.09)倍和(1.86±0.17)倍, 此作用可被渥曼青霉素(wortmannin)抑制. 胰岛素刺激了此2种细胞中的Akt磷酸化. L6-GLUT1myc肌原细胞的葡萄糖摄取量对胰岛素浓度呈剂量依赖性, 但与野生型细胞相比, 其对胰岛素的敏感性和最大响应没有改变. 但L6-GLUT4myc肌原细胞的葡萄糖摄取量对胰岛素的敏感性和最大响应均增加. 以前的研究提示毛喉素(forskolin)可能影响胰岛素刺激的GLUT4转位. 本研究表明, 在L6-GLUT4myc细胞中, 毛喉素使胰岛素刺激的葡萄糖摄取减少了65%, 此作用是由它对GLUT4的直接抑制而不是由其对GLUT4转位的影响造成的. 毛喉素和dipyridamole对GLUT4比对GLUT1有更强的抑制作用, 而戊巴比妥(pentobarbital)对GLUT1的抑制作用强于GLUT4. 应用这些抑制剂的结果表明、L6肌原细胞中基础状态下和胰岛素刺激状态下的葡萄糖主要由过表达的GLUT1或GLUT4转运. 因此, L6-GLUT1myc和L6-GLUT4myc细胞株为筛查对肌肉细胞GLUT1或GLUT4的活性或转位有不同作用的化合物提供了一个平台.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.003 |
| Insufficient payload (model declined to judge) | 0.011 | 0.007 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".