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Record W3145668878 · doi:10.1155/2021/6691402

The CD36 Ligand‐Promoted Autophagy Protects Retinal Pigment Epithelial Cells from Oxidative Stress

2021· article· en· W3145668878 on OpenAlexafffund
Marie‐France Dorion, Mukandila Mulumba, Shuya Kasai, Ken Itoh, William D. Lubell, Huy Ong

Bibliographic record

VenueOxidative Medicine and Cellular Longevity · 2021
Typearticle
Languageen
FieldMedicine
TopicRetinal Diseases and Treatments
Canadian institutionsUniversité de MontréalMcGill UniversityCegep Edouard MontpetitMontreal Neurological Institute and Hospital
FundersFonds de Recherche du Québec - SantéJapan Society for the Promotion of ScienceCanadian Institutes of Health ResearchNatural Sciences and Engineering Research Council of CanadaMitacs
KeywordsAutophagyCD36Oxidative stressCell biologyPigmentRetinalRetinal pigment epitheliumChemistryLigand (biochemistry)Oxidative phosphorylationBiologyApoptosisBiochemistryReceptor

Abstract

fetched live from OpenAlex

The retinal pigment epithelium (RPE) performs many functions that maintain photoreceptor health. Oxidative damage to the RPE is a critical component in the pathogenesis of eye diseases such as age‐related macular degeneration (AMD). Ligands of the cluster of differentiation 36 (CD36) have previously preserved photoreceptor integrity in mouse models of AMD. The cytoprotective effect of the CD36 ligand MPE‐001 on RPE cells has now been elucidated employing a model of oxidative stress. Sodium iodate (NaIO 3 ) induced formation of reactive oxygen species and apoptosis in human RPE cells, which were decreased by MPE‐001 without affecting antioxidant enzyme transcription. Immunoblotting and immunostaining assays showed a restorative effect of MPE‐001 on the autophagic flux disrupted by NaIO 3 , which was associated with an increase in syntaxin 17‐positive mature autophagosomes. The cytoprotective effect of MPE‐001 was completely abolished by the autophagy inhibitors wortmannin and bafilomycin A1. In conclusion, we report for the first time an autophagy‐dependent protection of RPE cells from oxidative stress by a CD36 ligand.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.031
Threshold uncertainty score0.997

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.019
GPT teacher head0.268
Teacher spread0.249 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations14
Published2021
Admission routes2
Has abstractyes

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