Bibliographic record
Abstract
Objective To identify and clone the gene encoding human ubiquitin binding enzyme and study its expression spectrum. Methods According to the sequence of human EST,which is highly homologous to the mouse ubiquitin conjugating enzyme(E2),primers used for library screening were synthesized to screen the human fetal brain cDNA library.The gene was analyzed by making use of bioinformatics and its expression spectrum was studied by using multiple tissue Northern blot. Results Two cDNA clones encoding human ubiquitin conjugating enzyme were isolated and identified.Both containing the ubiquitin conjugating domain,they were 88% identical in amino acid sequences and found to be isoforms of each other with only an exon excised from the short sequence.They belonged to a highly conserved,and widely expressed E2 enzyme family. Northern blot showed that they were expressed exclusively in heart,placenta,and pancreas while no transcripts could be detected in brain,lung,liver,skeletal muscle,or kidney. Conclusions The gene encoding human ubiquitin binding enzyme is expressed under both temporal and spatial control.As a key enzyme in the degradation of proteins,ubiquitin conjugating enzymes play a central role in the expression regulation on the level of post translation.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".