MétaCan
Menu
Back to cohort

Detection of Neuron Specific Enolase (NSE) with the Protein Biosensor Based on Imaging Ellipsometry

2012· article· en· W3149291390 on OpenAlex
Yu Niu, ZiyanZhao, Gang Jin

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

venuePublished in a venue whose home country is Canada.
no affNo Canadian affiliation: this work is invisible to an affiliation-only frame.
No Canadian affiliation. An affiliation-only frame, the usual design, would never have seen this work. It is one of the works that make the case for inverting the frame.

Bibliographic record

VenueJournal of Analytical Oncology · 2012
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicAdvanced Biosensing Techniques and Applications
Canadian institutionsnot available
FundersNational Key Research and Development Program of China
KeywordsEnolaseReproducibilityDetection limitBiosensorCalibration curveImmunoassayMolecular biologyGold standard (test)Biomedical engineeringAnalytical Chemistry (journal)ChemistryAntibodyMedicineMaterials scienceChromatographyPathologyNanotechnologyBiologyImmunohistochemistryRadiologyImmunology

Abstract

fetched live from OpenAlex

Tumor markers can provide convincing evidence for tumor angiogenesis in early-stage, so that the need for novel and effective methods which can detect tumor markers rapidly, sensitively and reliably is consequently being subjected to extensive interest. The biosensor based on imaging ellipsometry (BIE) is developed for the detection of Neuron specific enolase (NSE) as a trial and its diagnosis performance is evaluated. Anti-NSE antibody as ligand is immobilized on protein A modified silicon substrate to form NSE sensing surface. Then, NSE test is carried out with the setup of a calibration curve for clinical quantitative detection purpose. The relationship between BIE signal y (grayscale value) and NSE concentration x (ng/ml) is y=19.6 lg(x) + 70.1 and the limit of detection achieves 2 ng/ml. The specificity, reproducibility and accuracy for NSE detection with BIE are all adequate to clinical diagnosis requirements. 149 serum samples have been detected quantitatively with BIE and their results are in agreement with a commercial ELISA immunoassay.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.148
Threshold uncertainty score0.227

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.013
GPT teacher head0.286
Teacher spread0.273 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it