Bibliographic record
Abstract
类黄酮-3-O-葡萄糖基转移酶（3GT）能催化无色的花色素生成有色的花色苷。本研究采用RT-PCR法从彩色马铃薯中克隆了3GT基因,并对其进行了生物信息学分析和组织表达模式分析。结果显示,3GT基因编码448个氨基酸残基,具有信号肽但无跨膜结构域,还有一定的亲水性,定位于细胞质。同源比对表明3GT与茄子等茄科植物同属一簇,亲缘关系最近,与玉米等单子叶植物亲缘关系较远。3GT蛋白主要的二级结构元件为α-螺旋和无规则卷曲,且只有一个功能结构域,即氨基酸序列中的93~407区段,它与UDP-glucoronosyl、UDP-glucosyl transferase的功能结构域相匹配,因此,3GT属于UDPGT型糖基转移酶超家族的一员。组织特异性表达结果表明：3GT相对表达量和花色苷含量均是块茎高于叶片,它们的变化趋势基本一致,花色苷含量较高的器官,其3GT的相对表达量也较高,说明花色苷的积累与3GT的表达正相关。本研究可为花色苷积累的生化及分子机制研究奠定基础,并为进一步研究该基因在花色苷合成途径中的调控功能提供理论依据。
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.002 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.003 | 0.003 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.007 | 0.003 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".