Effects of Thermal Stability on Secretion of Methyl-parathion Hydrolase in Pichia pastoris
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Methyl parathion hydrolase can biodegrade organophosphorus pesticides. Due to its special application value,researchers paid more and more attension. Wild-type MPH-Och from Ochrobactrum sp. could not be secreted in Pichia pastoris. In order to increase the secretion level,the MPH-S274 Q mutant,which thermal stability was increased,was expressed in Pichia pastoris. After recombinant strain was induced by shaking culture flask for 120 h,the enzyme activity of culture supernatant reached 0. 7 U/m L. It was 14 times of that in wild type MPH-Och. The SDS-PAGE electrophoresis showed that the culture supernatant of mutant MPH-S274 Q had a distinct protein band,which further proved the improvement of secretion efficiency. This study indicated that improving protein thermal stability can be an effective way to increase secretion efficiency of exogenous protein in Pichia pastoris.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it