Effects of Thermal Stability on Secretion of Methyl-parathion Hydrolase in Pichia pastoris
Bibliographic record
Abstract
Methyl parathion hydrolase can biodegrade organophosphorus pesticides. Due to its special application value,researchers paid more and more attension. Wild-type MPH-Och from Ochrobactrum sp. could not be secreted in Pichia pastoris. In order to increase the secretion level,the MPH-S274 Q mutant,which thermal stability was increased,was expressed in Pichia pastoris. After recombinant strain was induced by shaking culture flask for 120 h,the enzyme activity of culture supernatant reached 0. 7 U/m L. It was 14 times of that in wild type MPH-Och. The SDS-PAGE electrophoresis showed that the culture supernatant of mutant MPH-S274 Q had a distinct protein band,which further proved the improvement of secretion efficiency. This study indicated that improving protein thermal stability can be an effective way to increase secretion efficiency of exogenous protein in Pichia pastoris.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".