3D Bioprinting Human‐Induced Pluripotent Stem Cells and Drug‐Releasing Microspheres to Produce Responsive Neural Tissues
Why this work is in the frame
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Bibliographic record
Abstract
3D bioprinting can produce complex human tissue mimics using stem cells (SCs). Herein, cylindrical constructs containing human‐induced pluripotent stem cell (hiPSC)‐derived neural progenitor cells (NPCs) encapsulated in a fibrin‐based bioink containing polycaprolactone (PCL)–retinoic acid (RA) and purmorphamine (puro)‐releasing microspheres are bioprinted in a layer‐by‐layer fashion using the microfluidic‐based RX1 bioprinter to engineer responsive neural tissues. The differentiated constructs contain neurons expressing ChAT, GABA, and MAP2, astrocytes expressing GFAP, and oligodendrocytes expressing O4 as indicated by immunocytochemistry and flow cytometry analysis on days 30 and 45. The bioprinted tissues also respond to treatment with acetylcholine (Ach) and gamma‐aminobutyric acid (GABA) on days 30 and 45. The use of microsphere‐laden bioinks efficiently promotes neural tissue differentiation and maturation in situ using a lower amount of morphogens in comparison with using soluble drugs. This bioprinting strategy serves as a cost‐effective solution for engineering humanized neural tissues.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.003 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it