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Record W3153314201 · doi:10.24908/iqurcp.10701

21. Making the World a Colourful Place: Exploration of the Key Condensation Enzymes in the Biosynthesis of Prodiginines and Tambjamines

2018· article· en· W3153314201 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

venuePublished in a venue whose home country is Canada.
no affNo Canadian affiliation: this work is invisible to an affiliation-only frame.
No Canadian affiliation. An affiliation-only frame, the usual design, would never have seen this work. It is one of the works that make the case for inverting the frame.

Bibliographic record

VenueInquiry Queen s Undergraduate Research Conference Proceedings · 2018
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMicrobial Metabolism and Applications
Canadian institutionsnot available
Fundersnot available
KeywordsBiosynthesisMoleculeEnzymeBacteriaCombinatorial chemistryChemistrySubstrate (aquarium)StereochemistryBiochemistryBiologyOrganic chemistryGenetics

Abstract

fetched live from OpenAlex

Bacteria produce many natural products that have useful bioactivities such as antibiotic, anticancer and antifungal effects. In bacteria, these molecules are made in a step-by-step process using proteins called enzymes that assist in building the molecule at each step of the process. This research is focusing on the biosynthesis of two classes of natural products called prodiginines and tambjamines. The molecules in each class have different structures but follow similar construction steps. Both processes first form the same common intermediate, then in the final step this intermediate is combined with another unique intermediate to make the final product. In this study, the enzyme that is involved in the final step of the process will be characterized for both prodiginine and tambjamine-type molecules. By purifying the enzymes for both types of molecule properties such as their catalytic mechanism, substrate flexibility, and structure can be determined and compared.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.117
Threshold uncertainty score0.516

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.090
GPT teacher head0.367
Teacher spread0.276 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it