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Record W3156595769 · doi:10.24908/iqurcp.11657

Gene Expression Analysis in Critically Ill Patients

2018· article· en· W3156595769 on OpenAlexvenueno aff
Mary Hoekstra

Bibliographic record

VenueInquiry Queen s Undergraduate Research Conference Proceedings · 2018
Typearticle
Languageen
FieldNursing
TopicInfant Nutrition and Health
Canadian institutionsnot available
Fundersnot available
KeywordsLactoferrinGene expressionGeneCritically illInnate immune systemBiologyGene expression profilingImmune systemImmunologyMedicineComputational biologyBioinformaticsGeneticsInternal medicine

Abstract

fetched live from OpenAlex

Nosocomial infections are associated with increased morbidity and mortality in intensive care units (ICUs) and remain an important target for intervention. Several studies have explored bovine lactoferrin as a potential tool for preventing infection and modulating the immune response. One clinical trial, the PREVAIL study, investigated the efficacy of lactoferrin in preventing nosocomial infections in critically ill, mechanically ventilated patients. A subset of patients in this study had blood samples collected at up to 6 different time points during their stay in the ICU, which were used to extract RNA for gene expression profiling. The resulting gene expression data were analyzed to determine the impact of lactoferrin on gene expression. Differential expression analysis was performed using both a single-gene approach and weighted gene co-expression network analysis (WGCNA). A single-gene analysis revealed that there was an increased number of differentially expressed genes in the lactoferrin group at all time points, including genes associated with the inflammatory response. Results from WGCNA revealed that groups of genes associated with innate immunity and defence response to virus were significantly differentially expressed over time in the lactoferrin group. These results contribute to our understanding of critical illness at the molecular level and provide evidence that lactoferrin has a biological effect.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.002

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.093
GPT teacher head0.405
Teacher spread0.312 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2018
Admission routes1
Has abstractyes

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