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Record W3157949170 · doi:10.24908/iqurcp.7743

Discovery-driven Science: a New Model for Cell Motility

2017· article· en· W3157949170 on OpenAlexvenueno aff
Eleasa Sieh

Bibliographic record

VenueInquiry Queen s Undergraduate Research Conference Proceedings · 2017
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMicrotubule and mitosis dynamics
Canadian institutionsnot available
Fundersnot available
KeywordsKinesinMicrotubuleMotor proteinCell biologyCytoskeletonMolecular motorBiologyProcessivityMotilityBiophysicsChemistryBiochemistryCellEnzyme

Abstract

fetched live from OpenAlex

Movement is an essential characteristic of life. On the cellular level, it is powered by many different classes of motor proteins. Kinesins are one class of linear molecular motor proteins, which move on cytoskeletal tracks called microtubules. Conventional kinesins are dimeric molecules composed of several domains, including a motor domain which has a catalytic core that binds to a nucleotide (ATP or ADP). The nucleotide-bound state of kinesin during the motile cycle affects its neck position, a determinant of its direction of movement, and microtubule affinity. There are two types of motor protein movement: processive, in which the motor steps progressively along the cytoskeletal track without detaching, and nonprocessive, in which the motor detaches from the track after a single power stroke. Conventional kinesins move processively due to the dimerization of the two motor heads. The two heads are kept out of phase due to communication between the heads. Kar3, the kinesin of interest in this project, moves non-processively and is the only C-terminal motor protein found in budding yeast. Its functional form is a heterodimer with one of two non-catalytic polypeptides, Cik1 and Vik1. This project showcases Vik1 as the key to a novel model for motility due to the surprising discovery that the Vik1:microtubule interaction is influenced by the nucleotide-binding state of Kar3. There must be communication between Kar3 and Vik1 to cause Vik1’s release from the microtubule, as well as to allow the progression of microtubule-binding and, thus, movement along the microtubule.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.003
metaresearch head score (Gemma)0.005
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.009
Threshold uncertainty score0.034

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0030.005
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.002
Bibliometrics0.0020.002
Science and technology studies0.0020.013
Scholarly communication0.0060.015
Open science0.0030.005
Research integrity0.0050.006
Insufficient payload (model declined to judge)0.0090.003

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.103
GPT teacher head0.391
Teacher spread0.287 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2017
Admission routes1
Has abstractyes

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