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Record W3158319663 · doi:10.1242/jeb.235366

Zebrafish can't take the heat around ‘safer’ BPAs

2021· article· en· W3158319663 on OpenAlexaff
Brittney G. Borowiec

Bibliographic record

VenueJournal of Experimental Biology · 2021
Typearticle
Languageen
FieldEnvironmental Science
TopicEffects and risks of endocrine disrupting chemicals
Canadian institutionsWilfrid Laurier University
Fundersnot available
KeywordsZebrafishPollutantFish <Actinopterygii>Bisphenol ASAFERHarmBisphenolPollutionToxicologyZoologyPhysiologyBiologyEnvironmental chemistryChemistryEcologyFisheryPsychologyBiochemistryComputer science

Abstract

fetched live from OpenAlex

Pollution is, unfortunately, a permanent fixture of many environments. One particularly concerning pollutant is bisphenol A (BPA), a chemical that leaches out of plastic and can harm animals by mimicking the sex hormone oestrogen. Fortunately, BPA is being replaced increasingly in our plastics by supposedly ‘safer’ alternatives, such as bisphenol S (BPS) and bisphenol F (BPF). However, there are concerns that bisphenol pollutants can affect how animals respond to changing environments. How pollution and climate change – arguably humanity's two greatest legacies on Earth – interact remains a mystery. Nicholas Wu and Frank Seebacher from The University of Sydney, Australia, set out to understand how exposure to these common, physiology-modifying pollutants affected how fish performed when they turned up the heat.Wu and Seebacher exposed zebrafish to an environmentally relevant level of BPA (30 μg l–1), or one of its alternatives, in chilly (18oC) and warm (28oC) water. Then they checked how fast the fish could swim at both temperatures to get a sense of how the bisphenols interfered with the fish's ability to perform in changing environments. The BPA-exposed fish swam fine, but the fish exposed to the other bisphenols couldn't keep up: BPF and BPS tanked the top speed that all of the fish could attain. In short, it turned out that the ‘safer’ BPAs are not always less toxic.To investigate how BPF or BPS exposure slowed the fish down, the researchers turned their attention to two metabolic enzymes linked to performance: citrate synthase and lactate dehydrogenase. Citrate synthase exists in the mitochondria, which provide power to all tissues. Animals with more citrate synthase have more mitochondria and more metabolic power for exercise. Lactate dehydrogenase is also involved in exercise and sometimes in adaptation to warm or cold environmentsHow the bisphenols affected citrate synthase activity depended on both a fish's previous experience (if they were warm or cold when exposed to the chemicals) and the temperate at which they did their swim test, highlighting how pollution could hinder fish coping with the temperature fluctuations that occur naturally in the environment. Wu and Seebacher found that when the fish were swimming in warm (28oC) water, BPA increased their citrate synthase activity, but the enzyme was less active in the fish that had been transferred from cold to hot water. When the duo compared the effects of BPF on the enzyme, they found the opposite: higher citrate synthase activity in the fish from cold water and lower in the warm ones. In contrast, BPS reduced the enzyme's activity regardless of temperature. As the researchers had found when testing the fish's swimming abilities, BPS and BPF were just as, if not more, disruptive than the compound they were created to replace, despite their ‘less toxic’ reputation.The bisphenols also impacted lactate dehydrogenase activity, but the effect depended somewhat on temperature. BPA decreased lactate dehydrogenase activity in cold fish, but the plastic increased the enzyme's activity in the warm fish, following a similar pattern as citrate synthase. BPF and BPS exposure reduced lactate dehydrogenase activities in all cases except for the fish from and swimming in warm water after a BPF exposure, which saw an increase in lactate dehydrogenase activity instead. Just like with citrate synthase, BPS and BPF were just as, if not more, metabolically disruptive than the infamous BPA.Pollutants such as BPA and its replacements are common in many habitats and affect many physiological processes, such as metabolism, which are important for dealing with environmental challenges. Though fish exposed to bisphenols can still obtain the same top speed, they have a lot of idiosyncratic differences under the hood that limit their ability to rise to thermal challenges and beat the heat of climate change.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.011
Threshold uncertainty score0.022

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.001
Bibliometrics0.0000.000
Science and technology studies0.0010.001
Scholarly communication0.0000.001
Open science0.0010.001
Research integrity0.0010.002
Insufficient payload (model declined to judge)0.0050.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.014
GPT teacher head0.337
Teacher spread0.323 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2021
Admission routes1
Has abstractyes

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Same venueJournal of Experimental Biology→Same topicEffects and risks of endocrine disrupting chemicals→French-language works237,207→