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Record W3158465197 · doi:10.1002/cnma.202100064

Hydroxyapatite Growth on Amelogenin‐Amelotin Recombinamers

2021· article· en· W3158465197 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueChemNanoMat · 2021
Typearticle
Languageen
FieldMedicine
TopicBone and Dental Protein Studies
Canadian institutionsUniversity of Toronto
FundersNatural Sciences and Engineering Research Council of Canada
KeywordsAmelogeninEnamel paintChemistryMineralization (soil science)Mineralized tissuesAmelogenesisBiomineralizationCell biologyBiophysicsCrystallographyBiochemistryDentistryAmeloblastGeologyBiologyGenePaleontologyMedicine

Abstract

fetched live from OpenAlex

Abstract Strategies to improve hydroxyapatite mineralization for enamel repair are essential for tissue regeneration. Amelogenin and amelotin (AMTN) are enamel matrix proteins playing critical roles in enamel formation. Amelogenin acts as a scaffold for hydroxyapatite, while AMTN (specifically its ‘SSEEL’ domain) is necessary for proper enamel mineralization. The functional relationship between recombinant AMTN and amelogenin, and their combined ability to guide uniaxial hydroxyapatite growth in vitro has been investigated recently. However, incorporation of the active domain of AMTN within recombinant amelogenins has not been studied yet. Here we describe the synthesis of modified amelogenin by inserting, internally and at its C‐terminus, the mineralizing AMTN‐derived motif SSEEL. C‐terminus modified amelogenin promoted hydroxyapatite formation, whereas internal incorporation of the motif initially resulted in amorphous calcium phosphate formation. Here we show that modified amelogenin with the mineralizing AMTN‐motif SSEEL can promote hydroxyapatite growth. These results give new insights for mineralized tissue regeneration using recombinamer proteins.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesInsufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.376
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.015
GPT teacher head0.251
Teacher spread0.236 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it