Effect of NFE2L1 Overexpression and Knock Down on the Response of XBP1 Splice Variants to Endoplasmic Reticulum Stress
Bibliographic record
Abstract
The unfolded protein response (UPR) is responsible for the degradation and refolding of misfolded proteins.Nuclear factor erythroid-2 like-1 (NFE2L1) basic leucine zipper (bZIP) transcription factor which respond primarily to oxidative stress and proteasome inhibition.In its role in maintaining proteostasis, NFE2L1 upregulates production of proteasomal subunits.Over the years, functional similarities and parallel response pathways have been observed between the UPR and NFE2L1.NFE2L1 may be involved in maintaining proteostasis via involvement in the UPR, directly.The activation of XBP1, a UPR effector, was analyzed under NFE2L1 knock down and overexpression.At the protein level, NFE2L1 knock down decreases the amount of activated XBP1 produced when treated with thapsigargin.NFE2L1 overexpression increased activated XBP1 synthesis under untreated conditions.NFE2L1 knock down resulted in significantly disrupted splicing of XBP1 RNA into its active form.These results suggest that NFE2L1 expression has an impact on UPR signaling.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".