DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR THE DETERMINATION OF ANDROGRAPHOLIDE FROM ANDROGRAPHIS PANICULATA (WHOLE PLANT)
Bibliographic record
Abstract
A simple, rapid, selective and quantitative HPTLC method has been developed for determination of Andrographolide in different samples of Andrographis paniculata (Whole Plant). The alcoholic extract of Andrographis paniculata (Whole Plant) samples were applied on TLC Aluminium plate pre coated with Silica gel60 GF254 and developed using Toluene : Ethyl acetate : Formic acid (5:4.5:0.5) v/v as a mobile phase. The plate was sprayed (derivatized) with Anisaldehyde‐ Sulphuric Acid reagent followed by heating at 1100C for 10 minutes and detection and quantification were carried out densitometrically using an UV detector at wavelength of 235 nm. Content of marker compound in the samples were found similar.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.002 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".