Clinical sensitivity and specificity of a quantitative PCR assay for bovine genital campylobacteriosis in bulls
Bibliographic record
Abstract
Bovine genital campylobacteriosis occurs worldwide wherever natural service is used. The disease is transmitted by carrier bulls and characterized by early pregnancy loss and infertility. Despite the challenge of maintaining viability of the Campylobacter fetus subsp venerealis (Cfv) during transport and avoiding overgrowth of contaminants, culture remains the "gold standard". Several PCR tests have been developed for the detection of Cfv, and primers VenSF/VenSR have been extensively tested and recognized worldwide. Recently, our research group at WCVM adapted these primers to a SYER Green qPCR platform. This assay presents a high analytical sensitivity and provides a fast and effective technique, suitable to be used as a screening test for the detection of carrier bulls. The objective of the present study was to determine the clinical sensitivity and specificity of the RT-PCR assay in direct preputial samples.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.004 | 0.007 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".