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Record W3165484892 · doi:10.1038/s41431-021-00852-7

Solving patients with rare diseases through programmatic reanalysis of genome-phenome data

2021· article· en· W3165484892 on OpenAlex
Carles Hernandéz-Ferrer, Davide Piscia, Enzo Cohen, Isabel Cuesta, Daniel Danis, Anne‐Sophie Denommé‐Pichon, Yannis Duffourd, Christian Gilissen, Steven Laurie, Shuang Li, Isabelle Nelson, Ida Paramonov, Prasanth Sivakumar, Peter N. Robinson, Karolis Sablauskas, Joeri K. van der Velde, Antonio Vitobello, Rebecca Schüle, Ana Töpf, Lisenka E.L.M. Vissers, Richarda de Voer, Stefan Aretz, Gabriel Capellá, Richarda M. de Voer, D. Gareth Evans, Elke Holinski‐Feder, Andreas Laner, Carla Oliveíra, Andreas Rump, Evelin Schröck, Anna Katharina Sommer, Verena Steinke‐Lange, Marc Tischkowitz, Laura Valle, Elisa Benetti, Giorgio Casari, Andrea Ciolfi, Bruno Dallapiccola, Elke de Boer, Kornelia Ellwanger, Laurence Faivre, Tobias B. Haack, Anna Hammarsjö, Markéta Havlovičová, Alexander Hoischen, Anne Hugon, Adam Jackson, Tjitske Kleefstra, Anna Lindstrand, Estrella López‐Martín, Milan Macek, Manuela Morleo, Vicenzo Nigro, Ann Nordgren, Maria Pettersson, Simone Pizzi, Manuel Posada, Francesca Clementina Radio, Alessandra Renieri, Caroline Rooryck, Lukáš Ryba, Martin Schwarz, Marco Tartaglia, Christel Thauvin, Annalaura Torella, Aurélien Trimouille, Alain Verloès, Pavel Votýpka, Klea Vyshka, Birte Zurek, Danique Beijer, Gisèle Bonne, Peter Hackman, Michael G. Hanna, Henry Houlden, Jarred Lau, Hanns Lochmüller, William L. Macken, Francesco Musacchia, A. Nascimento, Daniel Natera‐de Benito, Vincenzo Nigro, Giulio Piluso, Veronica Pini, Robert D. S. Pitceathly, Pedro M. Rodríguez Cruz, Anna Sarkozy, Rita Selvatici, Rachel Thompson, Liedewei Van de Vondel, Jana Vandrovcova, Irina Zaharieva, Patrick F. Chinnery, Alexandra Dürr, Tobias B. Haack, Holger Hengel, Christoph Kamsteeg, Katja Lohmann, Alfons Macaya, Ales Maver, Judit Molnar, Alexander Münchau, Borut Peterlin, Olaf Rieß, Lüdger Schöls, Rebecca Schüle, Giovanni Stévanin, Vincent Timmerman, Bart van de Warrenburg, Nienke van Os, Melanie Wayand, Carlo Wilke, Raúl Tonda, Marcos Fernandez-Callejo, Daniel Picó, Carles Garcia-Linares, Alberto Corvò, Ricky S. Joshi, Hector Diez, Marta Gut, Holm Graeßner, Stephan Ossowski, German Demidov, Marc Sturm, Julia M. Schulze‐Hentrich, Christoph Keßler, Peter Heutink, Han Brunner, Hans Scheffer, Peter A.C. ’t Hoen, Erik Janssen, Marloes Steehouwer, Burcu Yaldız, Anthony J. Brookes, Colin Veal, Spencer Gibson, Marc Wadsley, Mehdi Mehtarizadeh, Umar Riaz, Greg Warren, Farid Yavari Dizjikan, Thomas Shorter, Volker Straub, C. Marini Bettolo, Sabine Specht, Elizabeth Alexander, Laurence Faivre, Émilie Tisserant, Ange-Line Bruel, Christine Peyron, Aurore Pélissier, Leslie Matalonga, Gemma Bullich, Carles García, Gulcin Gumus, Virginie Bros‐Facer, Ana Rath, Marc Hanauer, Annie Olry, David Lagorce, Svitlana Havrylenko, Katia Izem, Fanny Rigour, Claire-Sophie Davoine, Léna Guillot‐Noël, Anna Heinzmann, Giulia Coarelli, Valérie Allamand, Rabah Ben Yaou, Corinne Métay, B. Eymard, António Atalaia, Tanya Stojkovic, Marek Turnovec, Dana Thomasová, Radka Pourová Kremliková, Věra Franková, Markéta Havlovicová, Vlastimil Kremlik, Helen Parkinson, Thomas Keane, Dylan Spalding, Alexander Senf, Glenn Robert, Alessia Costa, Christine Patch, Mary M. Reilly, Francesco Muntoni, Jonathan Baets, Peter De Jonghe, Sandro Banfi, Rachele Rossi, Marcella Neri, Isabel Spier, Carla Oliveira, Ana Rita Matos, Celina São José, Marta Ferreira, Irene Gullo, Susana Fernandes, Luzia Garrido, Pedro Ferreira, Fátima Carneiro, Morris A. Swertz, Lennart Johansson, Gerben van der Vries, Pieter B. Neerincx, Dieuwke Roelofs-Prins, Sebastian Köhler, Alison Metcalfe, Raffaele Castello, Alessandra Varavallo, Manuel Posada de la Paz, Eva Bermejo, Estrella López Martín, Beatriz Martínez Delgado, F. Javier Alonso García de la Rosa, Mária Judit Molnár, Katja Lohmann, Rebecca Herzog, Martje G. Pauly, Andres Nascimiento Osorio, David Beeson, Mridul Johari, Marco Savarese

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueEuropean Journal of Human Genetics · 2021
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenomics and Rare Diseases
Canadian institutionsUniversity of OttawaOttawa HospitalChildren's Hospital of Eastern Ontario
FundersFP7 HealthInstituto de Salud Carlos IIIMedical Research CouncilEuropean Regional Development FundHorizon 2020 Framework ProgrammeGeneralitat de CatalunyaMinisterio de Economía y CompetitividadAgence Nationale de la RechercheEuropean Commission
KeywordsPhenomeWorkflowExomeExome sequencingGenomeComputational biologyGenomicsBiologyComputer scienceBioinformaticsGeneticsDatabasePhenotypeGene

Abstract

fetched live from OpenAlex

Reanalysis of inconclusive exome/genome sequencing data increases the diagnosis yield of patients with rare diseases. However, the cost and efforts required for reanalysis prevent its routine implementation in research and clinical environments. The Solve-RD project aims to reveal the molecular causes underlying undiagnosed rare diseases. One of the goals is to implement innovative approaches to reanalyse the exomes and genomes from thousands of well-studied undiagnosed cases. The raw genomic data is submitted to Solve-RD through the RD-Connect Genome-Phenome Analysis Platform (GPAP) together with standardised phenotypic and pedigree data. We have developed a programmatic workflow to reanalyse genome-phenome data. It uses the RD-Connect GPAP's Application Programming Interface (API) and relies on the big-data technologies upon which the system is built. We have applied the workflow to prioritise rare known pathogenic variants from 4411 undiagnosed cases. The queries returned an average of 1.45 variants per case, which first were evaluated in bulk by a panel of disease experts and afterwards specifically by the submitter of each case. A total of 120 index cases (21.2% of prioritised cases, 2.7% of all exome/genome-negative samples) have already been solved, with others being under investigation. The implementation of solutions as the one described here provide the technical framework to enable periodic case-level data re-evaluation in clinical settings, as recommended by the American College of Medical Genetics.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.412
Threshold uncertainty score0.550

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.019
GPT teacher head0.244
Teacher spread0.225 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it