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Several PDE4‐family enzymes differentially regulate EPAC1‐mediated actions during VEC tubule formation

2013· article· en· W3166067655 on OpenAlexaff
Milosz Kaczmarek, Paulina Brzezińska, M. Bibiana Umana, Donald H. Maurice

Bibliographic record

VenueThe FASEB Journal · 2013
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicPhosphodiesterase function and regulation
Canadian institutionsQueen's University
Fundersnot available
KeywordsPhosphodiesteraseAngiogenesisCell biologyMatrigelChemistryPI3K/AKT/mTOR pathwayAdhesionPhosphodiesterase 3cAMP-dependent pathwayTubuleMAPK/ERK pathwayEnzymeBiologySignal transductionBiochemistryCancer researchEndocrinologyProtein kinase AKidney

Abstract

fetched live from OpenAlex

Studies have established that several angiogenesis‐related actions of vascular endothelial cells (VECs) are differentially affected by cAMP‐elevating agents. This selectivity is coordinated, in part, by actions of cAMP at individual VEC cAMP‐signaling complexes. Indeed, recently we showed that EPAC1‐integration into a phosphodiesterase 3B (PDE3B)‐based VEC cAMP‐signalosome allows PDE3 inhibitors to activate PI3Kã and promote VEC adhesion to matrigel, an early event in tubules formation. Since EPAC1 integrates into a PDE4D‐containing, VE‐cadherin‐based, cAMP signalosomes that allows EPAC1 to control VEC intercellular contacts, but not adhesions, we investigated how PDE4 inhibitors impacted VEC tubule formation. Our findings identify distinct roles for each PDE4B and PDE4D in the control of EPAC1‐mediated effects in tubule formation and identify potential novel therapeutic strategies to control these angiogenesis‐related events.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.131
Threshold uncertainty score0.362

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.213
Teacher spread0.202 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2013
Admission routes1
Has abstractyes

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