Impact of Coffee Containing Medium-Chain Triglyceride Oil and Ghee on Markers of Cellular Inflammation in Young Healthy Humans
Bibliographic record
Abstract
Low-carbohydrate, high-fat “ketogenic” food supplements have become increasingly popular in recent years with claims of improving body composition and cognition, while reducing hunger. However, acute consumption of high-fat foods has been shown to promote dietary endotoxemia; the release of bacterial lipopolysaccharide from the gut into the blood, which is linked to proinflammatory responses through activating toll like receptor (TLR) 4 on circulating monocytes. Bulletproof Coffee is a popular high-fat beverage consisting of coffee, medium chain triglyceride (MCT) oil, and grass-fed ghee. The purpose of this study is to determine whether consuming this high-fat coffee beverage would impact cellular inflammation assessed by increases in the number of circulating monocytes and monocyte surface TLR4 expression. We hypothesize that consuming one high-fat “Bulletproof Coffee” will elevate concentrations of circulating monocytes and increase TLR4 expression when compared to a black coffee comparator drink. This study is a single-blind (researcher), randomized crossover design wherein participants consume either a freshly prepared coffee (1 pod with 12oz water; ∼1 kcal), or high-fat bulletproof coffee (1 pod with 12 oz water containing 1 tbsp MCT oil and 1 tbsp ghee; 27 g fat; ∼250 kcal) separated by ∼7 days. Participants provided blood samples in the fasted state and at 60- and 180-minutes following beverage consumption. Blood samples were analyzed by flow cytometry. Six healthy adults (n = 5 females) aged 25 ± 8 years who consume coffee regularly have completed both conditions. Preliminary statistical analysis using a linear mixed model has shown no significant time x condition interaction (P = 0.184) or main effect of time (P = 0.211) for the concentration of circulating monocytes. Similarly, no interaction (P = 0.675) or main effects of time (P = 0.337) were observed for monocyte surface TLR4 expression. Preliminary data suggests that consuming a single high-fat bulletproof coffee does not appear to increase circulating monocyte concentrations or monocyte TLR4 expression. Further research will be required to determine whether acute consumption of a high-fat coffee beverage impacts inflammation in humans. This study is funded by a Natural Sciences and Engineering Research Council (NSERC) of Canada grant.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".