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Metabolomics profiling predicts SORT1 LDL‐cholesterol locus in a fit, young adult population

2013· article· en· W3167279257 on OpenAlexaff
Lawrence Kirtiadi, Elizabeth A. Gnatiuk, Angie Karlos, Kimberly Connors, Hans J. Vogel, Jane Shearer, Dustin S. Hittel

Bibliographic record

VenueThe FASEB Journal · 2013
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMetabolomics and Mass Spectrometry Studies
Canadian institutionsUniversity of Calgary
Fundersnot available
KeywordsMetabolomicsConfoundingLocus (genetics)GenotypeInternal medicinePopulationMetaboliteGeneticsGenome-wide association studyBiologyMedicineEndocrinologyBioinformaticsSingle-nucleotide polymorphismGene

Abstract

fetched live from OpenAlex

SORT1 locus was originally identified by genome wide association studies of LDL‐cholesterol (LDL‐C) in older adults. We hypothesized that a younger population would show a greater genetic effect due to fewer confounding variables. As such, we investigated the association between the SORT1 locus and LDL‐C in a group of healthy, young adults. Subjects (n=80, age=23) were recruited. Lipid measures and genomic DNA were collected from blood after an overnight fast. Blood pressure, body fat (%BF), V02 max, and metabolomics profile (LC‐MS) were measured. Associations between genotype and LDL‐C were investigated using linear regression. 21.7% of male subjects had %BF that was above a healthy range, while 25% had non‐optimal LDL‐C values. A significant association was observed between the SORT1 locus (GG: 2.46±0.11 mmol/L versus TG/TT: 2.06±0.12 mmol/L, p=0.016) and LDL‐C in male subjects with genotype explaining 3.0% of the variability in LDL‐C. LC‐MS metabolite profiling was able to further discriminate between genotypes independent of other clinical parameters. Differentially affected metabolites included sphingomyelins, phosphatidylcholines and acylcarnitines. The marriage of metabolomics and genomics represents a powerful means to identify the earliest biomarkers associated with cardiovascular disease that could be used to identify individuals who would most benefit from early interventions. Grant Funding Source : Metabolomics Research Center

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.006

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.237
Teacher spread0.225 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2013
Admission routes1
Has abstractyes

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