Nesfatin‐1 influences the excitability of subfornical organ neurons
Bibliographic record
Abstract
Nesfatin‐1, a centrally acting anorexigenic peptide, is produced in several brain areas involved in metabolic function and has been implicated in appetite and thirst reflexes. The present study was thus undertaken to determine the specific role of nesfatin‐1 in appetite and thirst regulation mediated by the subfornical organ (SFO). We first used RT‐PCR and were able to confirm the presence of nesfatin‐1 in SFO. We then used whole‐cell patch clamp recordings to investigate the influence of nesfatin‐1 on the membrane potential of dissociated SFO neurons. Approximately 68.8% (42 of 61) of neurons tested showed a response to nesfatin‐1 (10 nM and 1 nM). Of the responding neurons, 50% depolarized by a mean depolarization of 10.6 ± 1.64 mV (n=21) and 50% hyperpolarized by a mean hyperpolarization of −8.8 ± 2.2 mV (n=21). No effect was observed at a concentration of 100 pM (n=3). This study has demonstrated that nesfatin‐1 has the ability to change the membrane potential of SFO neurons, and therefore can be identified as a potential modulator of SFO function. Supported by the Canadian Institutes of Health Research
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".