Mechanisms of interferon gamma‐induced suppression of aquaporin 1 in murine intestinal epithelium
Bibliographic record
Abstract
Chronic diseases like IBD are characterized by high levels of IFNγ and abnormal intestinal transepithelial fluid flux. The water channel protein Aquaporin 1 (AQP1) is widely expressed on intestinal epithelial cells with the primary purpose of regulating transmembrane water flux. To investigate the deficiencies in water transport associated with IBD, we sought to determine the cellular signaling mechanisms by which IFNγ modulates AQP1 expression in the mouse intestinal epithelium. CMT93 cells treated with IFNγ significantly decreased constitutive AQP1 levels detected by immunofluorescence. Pre‐treatment with either the JAK1 inhibitor or STAT3 siRNA, prior to IFNγ administration resulted in the amelioration of the IFNγ‐induced suppression of AQP1 protein expression. Conversely, IRF‐2 siRNA pre‐treatment further decreased AQP1 protein levels following IFNγ treatment. Inflammatory cytokines present during IBD are able to influence AQP1 levels and may contribute to the water transport deficiencies associated with these disorders. In this study, the results suggest that decreased epithelial AQP1 expression exerted by IFNγ in CMT93 cells is regulated by JAK1 and STAT3. Of note, IRF‐2 which is normally considered to be a negative regulator of IFNγ signaling, acted in a positive regulatory fashion by further decreasing AQP1 protein signal. Source of funding: Crohn's & Colitis Foundation of Canada
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".