Phenylephrine‐induced calcium oscillations in human mesenteric arterial smooth muscle
Bibliographic record
Abstract
Phenylephrine (PE)‐induced oscillatory fluctuations in intracellular Ca 2+ concentration ([Ca 2+ ] i ) of vascular smooth muscle (SM) have been observed in every blood vessel isolated from a wide variety of mammals. Paradoxically, until recently similar observations in humans have proven elusive. In this study, we report for the first time observations of adrenergically‐stimulated [Ca 2+ ] i oscillations in human mesenteric artery SM. We partially characterized these oscillations by using the inhibitory drugs nifedipine, 2‐aminoethoxydiphenyl borate (2‐APB), cyclopiazonic acid (CPA), and HA‐1077. PE and one of these drugs were added to different arterial segments, which had previously been loaded with Fluo‐4 AM in PSS and mounted on a myograph on the stage of a confocal microscope. We observed PE‐induced oscillations in [Ca 2+ ] i , which initiated and maintained vasoconstriction. After PE contraction, all drugs provoked relaxation of the vessel segments, sometimes only partial, and reduced or inhibited oscillations, except CPA, which rarely caused relaxation. These preliminary results point to a potential involvement of the sarcoplasmic reticulum Ca 2+ and IP3R in the maintenance of the observed oscillations. FIS PI080920 , RECAVA RD06/0014/1007
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".