MITOCHONDRIAL DYSFUNCTIONS INDUCED BY OXIDATIVE STRESS IN CACO‐2CELLS
Bibliographic record
Abstract
Despite the fact that oxidative stress (OS) has been implicated in IBD pathogenesis and mitochondria are the substantial source of ROS, little attention has been paid to these organelles. We hypothesize that OS can lead to mitochondrial dysfunctions recapitulating various IBD abnormalities. The present study will to investigate the impact of OS on mitochondrial antioxidant content, energy production, calcium homeostasis, cell death, transcription factors status and DNA integrity. We used FE/ASC to generate OS in Caco‐2 cells. Our results show that the administration of FE/ASC (0.2 mM/2 mM) (1) increased MDA levels; (2) reduced ATP production; (3) led to alterations in the gene expression and protein mass of mitochondrial transcription factors (mtTFA, mTFB1, mTFB2) without any effects on POLRMT; (4) provoked dysregulation of mitochondrial calcium homeostasis; (5) elicited DNA lesions as illustrated by the raised levels of 8‐OHdG; (6) lowered, OGG1, one of the first lines of defence against 8‐oxodG mutagenicity; and (7) upregulated cytochrome C and AIF protein expressions, indicating exaggerated apoptosis. The presence of the powerful antioxidant BHT prevented the occurrence of OS and most of the mitochondrial abnormalities. In conclusion, our results suggest that OS causes mitochondrial dysfunctions and modulates mitochondrial transcription factors. We believe that the identification of OS‐mediated mitochondrion disorders adds new information regarding their implication in IBD pathogenesis.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".