Postnatal decreases in cardiac muscle fractional protein synthesis rates in fed pigs measured by an intraperitoneal flooding dose of L‐[ring‐2H5]Phe
Bibliographic record
Abstract
This study was conducted to quantify postnatal changes in cardiac muscle protein fractional synthesis rates (FSR) and their correlations with serum levels of hormones (nsulin, growth hormone, and cortisol), growth factors (IGF‐I and GLP‐2) and free amino acids (AA) in fed pigs. Thirty six purebred Yorkshire gilts were used for sampling at d 1, 4, 6, 12, 20 and 28 (1 wk post‐weaning) d of age. Piglets received ip injection of a flooding dose of Phe containing L‐[ring‐2H5] (40 molar%) in saline. Serum samples before the injection and plasma and heart samples at 30 min post‐injection were obtained for the determinations of tracer Phe enrichment by GC‐MS, free AA by HPLC, and hormones and growth factors by RIA. Tracer Phe enrichments in the plasma and cardiac muscle free AA pools were 25–27 molar%. Cardiac muscle FSR was 29.5, 28.5, 27.6, 28.9, 21.8, and 11.7 %/d at d 1, 4, 6, 12, 20, and 28 of age, respectively, with a linear decrease (P<0.05). Postnatal changes in FSR were negatively correlated (P<0.05, r=−0.41) with serum GLP‐2 levels. Postnatal changes in cardiac FSR were positively correlated (P<0.05) with the plasma concentrations of Asn, citrulline, His, Leu, ornithine, Ser, taurine, Tyr and Val (r=0.33–0.57) but negatively correlated (P<0.05) with Glu and Gly (r=−0.41 or −0.36). These results indicate dramatic postnatal decreases in cardiac muscle FSR from birth to the post‐weaning transition in fed pigs, which correlated well with changes in serum GLP‐2 and plasma several AA levels.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".