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Ultrastructural analysis of neurons, glia and capillaries in the peri‐infarct zone of an ischemic stroke

2013· article· en· W3173364348 on OpenAlexaff
Patrick C. Nahirney, Patrick Reeson, Craig E. Brown

Bibliographic record

VenueThe FASEB Journal · 2013
Typearticle
Languageen
FieldNeuroscience
TopicNeuroinflammation and Neurodegeneration Mechanisms
Canadian institutionsUniversity of Victoria
Fundersnot available
KeywordsPenumbraGlycogenBorder zoneStroke (engine)NeuropilPathologyAnatomyExcitatory postsynaptic potentialUltrastructureInfarctionSomatosensory systemBiologyChemistryMedicineIschemiaNeuroscienceInhibitory postsynaptic potentialCardiologyEndocrinologyCentral nervous systemMyocardial infarction

Abstract

fetched live from OpenAlex

Stroke is a serious condition that can drastically affect sensation, mobility and/or cognitive function. The peri‐infarct zone (or penumbra) is a hypo‐perfused region that, if re‐perfused, can regain function. To better understand this zone, mice were subjected to photothrombotic stroke in the forelimb somatosensory cortex (SSC) and allowed to recover for three days. Mice were then perfused and the SSC processed for light and electron microscopy. Infarcts appeared as swollen pale regions of the SSC that ranged in volume from 2–3mm 3 . Three zones were identified: the necrotic ischemic core, edematous, and structurally‐intact zones. In the necrotic zone, scattered erythrocytes, macrophages, neutrophils and eosinophils occupied the degenerating neuropil. The edematous zone (~100μm wide, adjacent to necrotic zone) exhibited swollen axons/dendrites 1–5μm wide, enlarged pyramidal neurons, glycogen‐filled astrocytes and capillaries with thickened and vacuolated endothelium. The intact zone (~200μm wide) contained dilated vessels surrounded by astrocytes with vast glycogen deposits. Excitatory and inhibitory synapses appeared structurally normal but reduced in number in the latter two zones. Understanding the cellular effects that occur in this zone will permit a more directed approach to therapies designed to reverse this damage. Supported by CIHR (CB), Heart & Stroke Foundation (CB) and NSERC (PN).

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.003

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.018
GPT teacher head0.240
Teacher spread0.223 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2013
Admission routes1
Has abstractyes

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