Multi‐tasking focal adhesion protein zyxin is present at tubulobulbar complexes
Bibliographic record
Abstract
Tubulobulbar complexes (TBCs) are actin‐related double‐membrane projections that form at intercellular junctions in the seminiferous epithelium of the mammalian testis. They form at basal junction complexes between neighboring Sertoli cells and apical junctions between Sertoli cells and germ cells. They are proposed to internalize intact junctions during sperm release and during the translocation of spermatocytes through basal junction complexes between neighboring Sertoli cells. Although TBCs are testis‐specific, they morphologically resemble podosomes in osteoclasts. Previously, we have reported that a complex of N‐WASp, Arp2/3, cortactin and dynamin that is found at podosomes also is present at TBCs. Here we demonstrate that zyxin, a focal adhesion protein known to be present at podosomes also is present at apical TBCs. A rabbit polyclonal anti‐zyxin antibody (B71) [generous gift from M.C. Beckerle, Huntsman Cancer Institute] was used to label fixed fragments and frozen sections of testis. Zyxin staining was intense in Sertoli cell regions known to contain tubular regions of TBCs. Zyxin is comprised of a proline rich N‐terminal domain and three LIM domains in its C‐terminal half. Although known to interact with the cytoplasmic domain of integrins, zyxin may be part of the mechanism of tubulobulbar complex formation. Supported by an NSERC Discovery Grant to AWV. Grant Funding Source : NSERC
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".