The binding and activation of Nitric Oxide Synthase by modified central linker and “phosphomimetic” Calmodulin proteins
Bibliographic record
Abstract
Calmodulin (CaM) is a ubiquitous calcium‐binding protein that is known to interact and regulate hundreds of intercellular functional proteins. The promiscuity of CaM is partially due to its highly flexible central linker region that separates its two terminal domains which interact with its target protein. Modification of the central linker region of CaM can result in changes in the proximity of the two terminal domains, their relative orientation, binding affinity and activation of the CaM target proteins. The present study was designed to investigate the role of the central linker of CaM by introducing select mutations, including phosphomimetic residues, into the CaM central linker region. The effects of these modifications on the binding and activation of the three mammalian nitric oxide synthase (NOS) isozymes have been investigated using biophysical techniques including circular dichroism, FTIR and gel shift mobility assays. Assays have been performed to assess the effect that modified CaM linker residues have on NOS enzyme activation and electron transfer. This research is supported by NSERC (J.G.G.)
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".