Serine protease‐induced decrease in epithelial ion permeability is dependent on an intact actin cytoskeleton
Bibliographic record
Abstract
The serine proteases trypsin, chymotrypsin and elastase induce a sustained increase in transepithelial electrical resistance (R TE ) when applied to the apical surface of polarised epithelial cell monolayers that is reflected by decreases in Na+ and Cl− paracellular permeabilities. Paracellular permeability is controlled at the intercellular tight junctions and the adherens junctions, with the epithelial actin cytoskeleton linked to proteins comprising both. We show that manipulation of the actin cytoskeleton with latruculin B and jasplakinolide reduces the protease‐induced increase in R TE by 92 ± 5 and 50 ± 8% respectively. Immunocytostaining for F‐ and G‐ actin indicate a shift in the filamentous actin pool. Inhibition of Rho kinases decreased baseline R TE but did not inhibit the protease‐induced increase in R TE .
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".