Clathrin‐associated Endocytic Proteins Eps15 and Epsin are Crucial for the Formation of Enteropathogenic E. coli (EPEC) pedestals
Bibliographic record
Abstract
Enteropathogenic E.coli (EPEC) are primarily extracellular pathogens that cause severe infantile diarrhea. The hallmark of EPEC pathogenesis is the generation of actin‐rich structures known as pedestals. Surprising evidence has revealed that while they persistently reside at an extracellular space, EPEC require the receptor‐mediated endocytosis protein, clathrin, for pedestal formation. In order to understand the strategies EPEC utilize to usurp endocytosis, we examined the roles of three major endocytic components: AP‐2, Eps15 and epsin, during pedestals formation. We found that pedestal formations required the recruitment of Eps15 and epsin, but not AP‐2. AP‐2 is known to provide linkage between Eps15, epsin and clathrin during the clathrin‐coated pit assembly process to complete endocytosis. Together, these results reveal a novel endocytic protein manipulation strategy employed by EPEC, supporting the recent paradigm that endocytic proteins are important for EPEC‐mediated disease. Grant Funding Source : CIHR
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".