CaMKII regulates intracellular calcium stores of native endothelial cells from mesenteric arteries
Bibliographic record
Abstract
Ca 2+ homeostasis is crucial for endothelial function and the regulation of vascular tone. The Ca 2+ ‐dependent kinase CaMKII is activated by Ca 2+ pulsars and might play an important role in endothelial Ca 2+ homeostasis. Thus, the involvement of endothelial CaMKII in the regulation of intracellular Ca 2+ dynamics has been investigated in native endothelial cells of mesenteric arteries. Acute exposure to KN‐93 (10 μM), a CaMKII inhibitor, has little if any effect on Ca 2+ pulsars frequency or amplitude. However, CaMKII inhibition induces pulsar sites recruitment as more pulsars sites are active in the presence of KN‐93 compared to control (25%). Interestingly, in absence of extracellular Ca 2+ , KN‐93 appears to empty intracellular Ca 2+ stores. Indeed, exposure to ionomycin, to empty endoplasmic reticulum in absence of extrallular Ca 2+ , provoked a large and transient intracellular Ca 2+ increase. However, preincubation with KN‐93 abolished the ionomycin‐induced Ca 2+ transient. These results suggest that CaMKII might inhibit IP 3 R receptors and/or phosphorylates phospholamban to regulate ER Ca 2+ content. Confocal microscopy revealed that CaMKIIα & β are colocalized with IP 3 R1 in native endothelium. Our data suggest that CaMKII is an important regulator of endothelial Ca 2+ homeostasis and may represent a novel target for the treatment of pathologies like hypertension. Supported by FRSQ, CFI, CIHR and HSFC.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".