Expression and functional characterization of murine organic anion transporting polypeptide 1b2 (oatp1b2/oatp4/lst‐1)
Bibliographic record
Abstract
The organic anion transporting polypeptide family plays important roles in mediating the cellular uptake of endogenous and exogenous compounds. Human liver enriched transporters such as OATP1B1 and OATP1B3 have been widely studied and shown to be involved in hepatic uptake and disposition of hormones, bile acids, and drugs. However, little is known about the murine orthologue, oatp1b2. Accordingly, expression of mouse oatp1b2 was assessed using real‐time PCR and Western blot analysis. mRNA transcripts were detected in a number of tissues including kidney, skeletal muscle, stomach and heart, with highest level in mouse liver. Immunohistochemical detection revealed localization of oatp1b2 in the sinusoidal membrane of hepatocytes. In contrast to the mRNA data, oatp1b2 protein expression was not detected in other tissues but liver. Cloning of the full coding region of oatp1b2 revealed the presence of two new splice variants. Interestingly, these variants were significantly expressed in the organs noted previously, but much less in liver. Heterologous expression experiments revealed taurocholic acid, estrone‐3‐sulfate, estradiol‐17β‐glucuronide, pravastatin and cerivastatin as substrates of the transporter. These findings give insights to species related differences in OATP transporter function that has application to mouse models of hepatic drug disposition.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".