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Role of prostaglandins in the loss of lymphatic contractile function in the experimental guinea pig model of TNBS‐induced ileitis

2009· article· en· W3175422022 on OpenAlexafffundabout
Sonia Rehal, Simon Roizes, Pierre‐Yves von der Weid

Bibliographic record

VenueThe FASEB Journal · 2009
Typearticle
Languageen
FieldMedicine
TopicLymphatic System and Diseases
Canadian institutionsUniversity of Calgary
FundersCrohn's and Colitis Foundation of Canada
KeywordsLymphatic systemProstaglandin E2 receptorProstacyclinIleumEndocrinologyInternal medicineDownregulation and upregulationProstaglandinProstaglandin EProstaglandin E2InflammationIleitisReceptorChemistryMedicineImmunologyBiochemistry

Abstract

fetched live from OpenAlex

The lymphatic vessel pumping is critical in maintaining tissue fluid homeostasis, particularly during edematous stress, such as inflammation. Loss of this function (i.e. dilated hyporesponsive lymphatic vessels) previously observed during experimental intestinal inflammation could be mediated by prostaglandins, which are heavily involved in the intestinal inflammatory response and potently alter lymphatic contractility. We thus evaluated the ability of lymphatics to synthesize and respond to prostaglandins in physiological and inflammatory conditions. Using real time PCR, we analyzed mesenteric lymphatics, arteries, veins, mesothelium and ileum obtained from control animals, TNBS‐treated guinea pigs and their sham counterpart for the following mRNA transcripts: COX‐1, COX‐2, prostacyclin synthase, prostaglandin E (PGE) synthase 1 and 2, prostacyclin receptor, and PGE 2 receptors EP1, EP2, EP3 and EP4. We also measured PGE 2 content in lymphatics by ELISA. When compared to sham and control animals, we observed an upregulation of COX‐2 mRNA and a downregulation of PGE synthase 2 and EP4 receptor mRNAs in lymphatics and ileum of TNBS‐treated animals. Preliminary data also showed an increase in PGE 2 content in lymphatics from TNBS‐treated animals. Our results further suggest alteration of PGE 2 function in lymphatics of TNBS‐treated guinea pigs Supported by the Crohn's and Colitis Foundation of Canada

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.508
Threshold uncertainty score0.187

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.025
GPT teacher head0.277
Teacher spread0.253 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2009
Admission routes3
Has abstractyes

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