Peroxynitrite Modulates The Expression of Gi Protein And Adenylyl Cyclase Signaling in Vascular Smooth Muscle Cells.
Bibliographic record
Abstract
We have further shown that vascular smooth muscle cells(VSMC) exposed to SNAP (NO donor)decreased the levels and functions of Giα proteins, which was restored to control levels by MnTBAP(peroxynitrite(ONOO‐) scavenger) and not by ODQ(soluble guanylyl cyclase inhibitor) suggesting that NO‐induced decreased Gi protein expression may be cGMP‐independent and attributed to the increased levels of ONOO‐. The present Studies were undertaken to investigate the role of ONOO‐ in the modulation of Gi protein expression in VSMC. A10 and aortic VSMC were used and treated with 3‐Morpholinosydnonimine hydrochloride (SIN‐1) or ONOO‐ for 24 hrs and the expression of Giα proteins was determined by immunoblotting techniques. SIN‐1 and ONOO‐ decreased the expression of Giα‐2 and Giα‐3 proteins in a concentration manner. The maximal inhibition of about 30% and 40 % was observed at 0.5mM SIN‐1 or at 0.5mM ONOO‐ in A‐10 and VSM cells respectively. The decreased expression of Giα proteins was reflected in decreased Gi functions. Angiotensin II(AngII) and C‐ANP4‐23– mediated inhibitions of adenylyl cyclase were significantly attenuated by SIN‐1 or ONOO‐ treatments. On the other hand, the expression of Gsα protein was not altered by these treatments in both types of cells, however, the stimulatory effects of isoproterenol, sodium fluoride (NaF) and forskolin (FSK) on adenylyl cyclase were significantly augmented. These results suggest that ONOO‐ decreased the expression of Gi proteins and associated functions in vascular smooth muscle cells. (supported by grant from Canadian Institutes of Health and Research)
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".