Small and intermediate conductance Ca <sup>2+</sup> ‐activated K <sup>+</sup> channels regulate spontaneous contraction of mouse portal vein myocytes
Bibliographic record
Abstract
Ca 2+ ‐activated K + channels (K Ca ) are key components of vascular myocytes EC coupling through their control of smooth muscle cell membrane potential. Whilst BK Ca is expressed ubiquitously in smooth muscle cells, only proliferative VSMCs express K Ca 3.1 channels. However, mouse portal vein (mPV) is an atypical blood vessel showing spontaneous and rhythmic contractions. Since K Ca channels are involved in the regulation of excitability of other cell types like neurons, the contribution of K Ca 2.x and K Ca 3.1 channels to the control of mPV excitability was investigated. Expression of K Ca 2.x and K Ca 3.1 was detected in mPV by PCR and immunohistochemistry. In single cell patch clamp studies, the RMP of isolated mPV myocytes was hyperpolarized (≈15 mV) by exposure to NS309 (10 μM), a K Ca 2.x and K Ca 3.1 channel activator. Inhibition of K Ca 2.x channels with UCL1689 (3 μM) reversed the effect of NS309 whilst inhibition of K Ca 3.1 channels (TRAM‐34; 10 μM) had no effect. NS309 significantly decreased the amplitude and increased the frequency of spontaneous contractions of intact mPV. These effects were reversed by application of UCL1689, suggesting that the NS309‐induced changes in spontaneous mPV contractions is carried out by K Ca 2.x channels. This study suggest that K Ca 2.x and K Ca 3.1 channels are expressed in mouse portal vein myocytes and play an important role in regulation of spontaneous contractions. Supported by FICM
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".