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A Role for CD34 in the Response of Skeletal Muscle to Hypoxic Conditions

2015· article· en· W3176611864 on OpenAlexaff
Melissa M. Page, Anick Langlois, Annie Dubé, Dany Patoine, François Maltais, Mathieu C. Morissette, Richard Debigaré, Marie‐Renée Blanchet

Bibliographic record

VenueThe FASEB Journal · 2015
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMuscle Physiology and Disorders
Canadian institutionsUniversité LavalInstitut universitaire de cardiologie et de pneumologie de Québec
Fundersnot available
KeywordsCD34Skeletal muscleHypoxia (environmental)WastingCOPDEndocrinologyMyogenesisInternal medicineMuscle atrophyGastrocnemius muscleBiologyAndrologyChemistryImmunologyMedicineCell biologyStem cellOxygen

Abstract

fetched live from OpenAlex

CD34, a satellite cell (SC) marker participates in skeletal muscle (SM) regeneration, facilitating the migration of SC. A fraction of patients with chronic obstructive pulmonary disease (COPD) develop SM atrophy, in part due to increased SC senescence. COPD patients experience transient hypoxemia, and it was suggested these hypoxic episodes may participate in SM wasting. Recently, we have observed that in vitro exposure of SC to hypoxic conditions increased CD34 expression and retained SC in an undifferentiated state. The goal of the present study was to evaluate the importance of CD34 expression in the muscle response to hypoxic conditions in vivo . Total weight and lean body mass were measured in WT and Cd34 knockout ( Cd34 ‐/‐ ) mice exposed to hypoxic conditions (10% O 2 ). The gastrocnemius expression of CD34 and genes involved in muscle regeneration were detected by qRT‐PCR. Results show an important loss of total weight and lean body mass at 2 days, which is significantly worse in Cd34 ‐/‐ mice. Alterations in Mrf4 and MHC expression were observed in Cd34 ‐/‐ mice vs. WT, suggesting a role for this protein in the response of muscle to hypoxic conditions. All in all, our results suggest that CD34 expression is important for the response of SM to hypoxic conditions, leading to a possible role in SM wasting in COPD.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.004

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.019
GPT teacher head0.284
Teacher spread0.264 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2015
Admission routes1
Has abstractyes

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