Absence of salt sensitivity in iNOS knockout mice
Bibliographic record
Abstract
The ability of aminoguanidine to promote salt‐sensitive hypertension has led to the suggestion that the inducible form of nitric oxide synthase (iNOS, NOS‐2) contributes importantly to the regulation of salt balance and blood pressure (BP). Here, we tested the hypothesis that iNOS knockout mice (iNOS‐/‐) have increased salt sensitivity of BP relative to control animals (iNOS+/+). Male mice of either strain were placed on regular (0.5% NaCl, RS) or high (8% NaCl, HS) salt diet at 9 wks, and 24h diastolic (DBP) and systolic (SBP) BP were assessed by telemetry after 3 months. In iNOS+/+ mice, 3 months of HS was associated with a modest elevation of BP compared to the RS group (RS vs HS: DAP, 91 ± 4 vs 98 ± 6 mmHg, P<0.05, SAP, 124 ± 2 vs 128 ± 5 mmHg, NS), consistent with previous studies. In contrast, there was no evidence of this effect of HS in iNOS‐/‐ mice (RS vs HS: DAP: 95 ± 4 vs 92 ± 2 mmHg, NS, SAP: 126 ± 4 vs 124 ± 6 mmHg, NS). The BP of iNOS‐/‐ and iNOS+/+ mice on RS diet were not significantly different. The BP response to brief (4 day) increases (RS to HS) or decreases (HS to RS) in salt intake did not differ between the two strains. In summary, our results demonstrate that iNOS knockout mice are normotensive and do not exhibit an exaggerated BP response to high salt. We reject our original hypothesis and conclude that absence of iNOS does not lead to an obligatory increase of salt sensitivity or BP. Supported by the Canadian Institutes of Health Research.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".