Gating of Pannexin 1‐Mediated ATP Release Channels by Mechanical Stress Stimuli
Bibliographic record
Abstract
Most cells release ATP to extracellular compartments wherein ATP acts as a signaling molecule to regulate responses such as vasodilation and inflammatory activation. Pannexin‐1 (Panx1) hemichannels have been proposed as major conduits for ATP efflux in response to proapoptotic stimulation or mechanical deformation of the plasma membrane. Recent studies have demonstrated that caspase‐mediated cleavage of the Panx1 C‐terminus is a novel gating mechanism for this channel in apoptotic cells. In this study we examine the possible role of caspases or other proteases in the acute ATP release induced by non‐apoptotic mechanical stress stimuli in rat C6 glioma cells (which lack native Panx1) versus C6 cells stably transfected with Panx1 (C6‐Panx1). C6 cells were mechanically stimulated by medium exchange or by thrombin (which triggers rapid reorganization of the actin cytoskeleton) to elicit acute ATP release. Only minor ATP release was observed in control C6 cells in response to either medium exchange or thrombin addition. In contrast, medium exchange triggered robust ATP release from C6‐Panx1 cells. Current experiments are testing whether thrombin triggers similar ATP release in C6‐Panx1 cells and whether proteolysis of Panx1 mediates gating of these ATP release channels in response to mechanical stimulation.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".