Generation of circulating globular adiponectin is catalyzed by thrombin
Bibliographic record
Abstract
The circulating levels of the cardiprotective adipokine adiponectin are reduced in obesity and diabetes. Lower adiponectin levels may contribute to the higher incidence of cardiovascular disease in these conditions. A truncated and biologically active form of adiponectin called globular adiponectin is protective against cardiovascular disease. However, little is known about its formation in vivo. Digestion in vitro with trypsin or leukocyte elastase produces a polypeptide with similar structure and biological activity to globular adiponectin. To address how globular adiponectin is produced endogenously, we developed an assay that measures cleavage of recombinant adiponectin into globular adiponectin by serum. N‐terminal sequencing of globular adiponectin revealed proteolysis occurred at Gly‐93. It was also found that the protease exclusively required Ca2+ for activity and that had an apparent molecular mass of about 80 kDa by gel filtration chromatography. The enzyme was insensitive to an inhibitor of leukocyte elastase, but was inhibited by hirudin and argatroban, specific inhibitors of thrombin. Our data indicate that thrombin likely mediates formation of globular adiponectin in the circulation. Supported by Canadian Institutes for Health Research.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".