Electrochemical Characterization of Carboplatin at Unmodified Platinum Electrodes and Its Application to Drug Uptake Studies in Ovarian Cancer Cells
Bibliographic record
Abstract
Epithelial ovarian cancer affects approximately 240,000 women worldwide with an estimated of 152,000 deaths per year. Common treatments include surgery or chemotherapy using a combination of carboplatin and taxane. Unfortunately, approximately 75% patients relapse within 18 months. Of these relapsed patient, an estimate of 85% experience carboplatin resistance. The effect is devastating. One way to combat this emerging drug resistance problem is the development of novel drugs. However, cellular mechanisms of carboplatin-resistance are poorly understood. Without a thorough understanding of resistance mechanisms, developing long-lasting effective drugs for long term treatment is a challenging problem. Therefore, there is a dire need to develop methods to detect and study resistance mechanisms. One suggested mechanism is the inhibition of drug uptake to prevent the intracellular accumulation of the drug. The presented work describes the quantification of carboplatin uptake in ovarian cancer cells. For this purpose, carboplatin is thoroughly characterized by voltammetry and its suitability for physiological bioelectrochemical studies is assessed. Diffusion and adsorption processes of carboplatin are evaluated, its dependence on pH is investigated, and a limit of detection for carboplatin at unmodified Pt electrodes is identified, without the need of expensive and time-intense electrode modification strategies. The influx and efflux of carboplatin into/from ovarian cancer cells are quantified by electrochemistry to better understand mechanisms of carboplatin chemoresistance. Results emerging from this research will be important for future medical research developing novel agents to combat chemoresistance and will present the basis for future long-term funding applications under this collaboration. Reference: Luu H.T.L., Nachtigal M.W., Kuss S.: Electrochemical Characterization of Carboplatin at Unmodified Platinum Electrodes and its Application to Drug Uptake Studies in Ovarian Cancer Cells. Journal of Electroanalytical Chemistry 2020, 872C, 114253.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".