Author Correction: Rare CASP6N73T variant associated with hippocampal volume exhibits decreased proteolytic activity, synaptic transmission defect, and neurodegeneration
Bibliographic record
Abstract
The original version of this Article contained an error in Fig. 7 g where the density maps in panel (g) were rendered incorrectly due to an error in the custom code. The different cell morphologies were not aligned properly, giving rise to a duplicated image and a widened impression in Fig. 7 g. The original Fig. 7 and accompanying legend appear below. Figure 7 Casp6N73T is less damaging to neuronal function and neurodegeneration than Casp6WT. ( a ) Representative two-photon images of 10 pg Casp6C163A- (Alexa 488, green) and 10 pg Casp6WT- or Casp6N73T- (Alexa 594, red) patched hippocampal CA1 pyramidal neurons. White arrows indicate basal dendrite degeneration. Scale bar: 25 µm. Maximum-intensity projection of two-photon stacks was compiled using ImageJ, and imaging montage of entire neurons was performed using Affinity Designer 1.7. ( b ) Sample EPSP time course plots from Casp6C163A- (green) and Casp6WT- (red) patched neurons in ( a ), showing reduction of neurotransmission for Casp6WT (1.53 ± 0.32 mV, n = 8 vs. 0.14 ± 0.07 mV, n = 8, p < 0.01) but not for Casp6C163A (1.94 ± 0.32 mV, n = 10 vs. 1.91 ± 0.29 mV, n = 10, p = 0.76) when comparing the last 10 traces (light thick line) to the first 10 traces (dark thick line). Open circles: EPSP amplitude recorded every 30 s. Closed circles: EPSP amplitude binned and averaged across 10 traces. Inset: representative EPSP traces highlight the paired-pulse ratio (PPR). Scale bars: 2 mV, 25 ms. Resting membrane potential and input resistance remained stable throughout experiment. ( c ) EPSP time courses for Casp6C163A- (n = 10), Casp6N73T- (n = 8), or Casp6WT- (n = 8) patched neurons. ( d ) PPR from Casp6WT-, Casp6C163A- or Casp6N73T-patched neurons. One-way ANOVA ( p = 0.0043), followed by Tukey’s post-hoc test (** p < 0.01 vs. C163A; # p < 0.05 vs. WT). ( e ) CV analysis of Casp6N73T- and Casp6WT-patched neurons. Casp6C163A was unaltered. ( f ) Representative reconstructions of Casp6C163A-, Casp6N73T-, or Casp6WT-patched neurons. Image stacks were used for manual reconstruction of 3D morphologies using the Neuromantic freeware ( http://www.reading.ac.uk/neuromantic/body_index.php ). ( g ) Dendritic density maps of Casp6C163A- (n = 11), Casp6N73T- (n = 8), or Casp6WT- (n = 8) patched neurons generated using custom software running in Igor Pro 8 v8.04 ( https://www.wavemetrics.com , https://github.com/pj-sjostrom/qMorph ). Dotted lines show the convex hull of the maximum extent. ( h ) Cumulative dendritic length of reconstructed neurons in layers SO, PCL, SR, and SLM. ( i ) Casp6C163A-, Casp6N73T- and Casp6WT-patched CA1 pyramidal neurons beading basal dendrites. One-way ANOVA ( p = 0.0001), followed by Tukey’s test (**** p < 0.0001 vs Casp6C163A, ## p < 0.01 vs Casp6WT). Full size image
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.019 |
| Meta-epidemiology (narrow) | 0.002 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.002 | 0.001 |
| Science and technology studies | 0.002 | 0.002 |
| Scholarly communication | 0.002 | 0.001 |
| Open science | 0.003 | 0.001 |
| Research integrity | 0.006 | 0.007 |
| Insufficient payload (model declined to judge) | 0.045 | 0.019 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".