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Record W3195263463 · doi:10.1101/2021.08.13.456086

The small and large intestine contain transcriptionally related mesenchymal stromal cell subsets that derive from embryonic <i> Gli1 <sup>+</sup> </i> mesothelial cells

2021· preprint· en· W3195263463 on OpenAlexaff
Simone Isling Pærregaard, Sophie Schussek, Line Wulff, Kristoffer Niss, Urs Mörbe, Johan Jendholm, Kerstin Wendland, Anna Andrusaite, Kevin Brulois, Robert J. B. Nibbs, Katarzyna Sitnik‐Warchulska, Allan McI. Mowat, Eugene C. Butcher, Søren Brunak, William W. Agace

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2021
Typepreprint
Languageen
FieldMedicine
TopicCancer Cells and Metastasis
Canadian institutionsInstitute of Infection and Immunity
FundersKnut och Alice Wallenbergs StiftelseH. Lundbeck A/SVetenskapsrådetLundbeckfondenUniversity of GlasgowScience for Life LaboratoryMemorial Sloan-Kettering Cancer CenterLeona M. and Harry B. Helmsley Charitable Trust
KeywordsBiologyMesenchymal stem cellEmbryonic stem cellCell biologyLamina propriaStromal cellHomeostasisSmall intestineCompartment (ship)ImmunologyGeneGeneticsEpitheliumCancer researchEndocrinology

Abstract

fetched live from OpenAlex

Summary Intestinal fibroblasts (FB) play essential roles in intestinal homeostasis. Here we show that the small and large intestinal lamina propria (LP) contain similar FB subsets that locate in specific anatomical niches and express distinct arrays of epithelial support genes. However, there were tissue specific differences in the transcriptional profile of intestinal FB subsets in the two sites. All adult intestinal LP mesenchymal stromal cells (MSC), including FB, smooth muscle cells (SMC) and pericytes derive from Gli1 -expressing embryonic precursors which we identify as mesothelial cells. Trajectory analysis suggested that adult SMC and FB derive from distinct embryonic intermediates, and that adult FB subsets develop in a linear trajectory from CD81 + FB. Finally, we show that colonic subepithelial PDGFRα hi FB comprise several functionally and anatomically distinct populations that originate from an Fgfr2 -expressing FB intermediate. Collectively our results provide novel insights into MSC diversity, location, function and ontogeny, with implications for our understanding of intestinal development, homeostasis and disease.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.009

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0030.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.013
GPT teacher head0.204
Teacher spread0.191 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations2
Published2021
Admission routes1
Has abstractyes

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