Mechanism of <i>Staphylococcus aureus</i> peptidoglycan <i>O</i> -acetyltransferase A as an <i>O</i> -acyltransferase
Bibliographic record
Abstract
Significance Enzymes comprised of membrane-spanning acyltransferase-3 (AT-3) domains catalyze the O-acetylation of diverse extracytoplasmic glycans in all forms of life. In many cases, such as peptidoglycan, these glycans represent important cell wall components, and their O-acetylation confers resistance to lytic enzymes. The enzyme responsible for peptidoglycan O-acetylation in gram-positive bacteria, OatA, is a single bimodal protein of an AT-3 domain fused to an SGNH domain. The AT-3 domain adopts a different topology to that predicted in silico. Moreover, its utilization of a unique mechanism for the translocation of acetyl groups across the cytoplasmic membrane for their transfer to peptidoglycan involving catalytic Tyr and Ser residues may be broadly applicable to homologs involved in the modification of other important cell wall glycans.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".