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Record W3197156127 · doi:10.1139/cjb-2021-0085

Evidence of starch accumulation in tobacco Bright Yellow (TBY-2) cells in the presence of auxin

2021· article· en· W3197156127 on OpenAlexvenueno aff
Veronica Ambrosini, Mohammad Issawi, Catherine Riou

Bibliographic record

VenueBotany · 2021
Typearticle
Languageen
FieldNursing
TopicFood composition and properties
Canadian institutionsnot available
FundersUniversité de Limoges
KeywordsAmylopectinStarchAmyloseCytokininBiologyFood scienceYield (engineering)PolysaccharideNicotiana tabacumBotanyAuxinBiochemistry

Abstract

fetched live from OpenAlex

Tobacco Bright Yellow (TBY-2) cell suspension is known to produce starch when cultured in a medium supplemented with cytokinin or in a hormone-free medium. Unexpectedly, TBY-2 cells continuously cultivated in the presence of auxin alone were also able to accumulate starch at the beginning of a stationary growth phase, with a yield of 9.22% ± 0.68%. This starch production was strongly correlated with a 25-fold increase in starch synthase activity. Moreover, this TBY-2 line was able to produce an amylopectin-rich starch with a ratio of amylopectin over amylose of 2.7, which was also linked to typical small granules (1.4 µm). According to our preliminary results, this plant cell suspension could yield a low-cost, amylopectin-rich starch — which is needed in the food industry to produce edible film or bioplastic — without being impacted by climate or seasonal weather changes.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.003

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.083
GPT teacher head0.324
Teacher spread0.241 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2021
Admission routes1
Has abstractyes

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