Micropropagation and Cryopreservation of Endangered Plant Species- Draba yukonensis and Cirsium hillii. (Canby) Fernald
Bibliographic record
Abstract
This study utilized in-vitro techniques for long-term conservation and revival of the threatened species, Hill’s Thistle (Cirsium hillii. (Canby) Fernald) and Yukon Draba (Draba yukonensis). Micropropagation protocols were developed from seed derived shoots. The cryopreservation protocol for in-vitro shoot tips of Hill’s thistle was established using the droplet-vitrification method which produced 90% shoot tips regrowth. The plants from cryopreserved shoot buds exhibited comparable or better survival and growth in the field compared to plants from non-cryopreserved shoot buds when transplanted to their natural habitats in the Bruce Peninsula National Park, Ontario. The Yukon Draba plants were successfully micropropagated and acclimatized in the greenhouse. Droplet-vitrification method used to optimize cryopreservation protocol for Yukon Draba using in-vitro shoot tips which resulted in 100 % survival and 66 % regrowth. Collectively, these results provide evidence of the successful application of in-vitro technologies for ex-situ conservation and recovery of endangered plant species.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".