Effect of Intracellular and Extracellular Mushroom Polysaccharides on Growth Inhibition of Human Carcinoma Cell Lines
Bibliographic record
Abstract
Introduction: Mushroom polysaccharides play an important role in nutraceutical and functional food because they act as biological active modifiers. The aim of the present work involved the production, purification and partial characterization of intracellular (IPS) and extracellular polysaccharides (EPS) from several basidiomycete strains. Such polysaccharides were used to investigate their effect on growth of human carcinoma cell lines. Methods: Mushroom polysaccharides were produced from several basidiomycete strains by submerged and solid state fermentations, assayed for superoxide radical scavenging activity, purified by gel filtration chromatography, analysed by FTIR and their effect on human carcinoma cell line was investigated by MTT method. Results: Mushroom polysaccharides have revealed scavenging activity in the range of 22 - 81 % for Po (s) and Pe (2), respectively. FTIR analysis of polysaccharides showed absorption bands characteristics of these biological macromolecules. IPS inhibited cell growth of HeLa in the range of 16.8 - 27.01 % for Po (s) and Ga (1), respectively. EPS inhibited cell growth of HeLa, A459, A431 and OE21 in the ranges of 3.08 - 92.2 %, 13.8 - 97.4 %, 14.7 - 93. 8% and 25 - 94% for Il (1) and Ga (1), Gc (1) and Ga (1), Il (1) and Ga (1), Le (1) and Ga (1), respectively. Purified preparations of polysaccharides confirmed the growth inhibition of these biomolecules. Conclusion: The present results strongly suggest growth inhibition of human carcinoma cell lines by mushroom polysaccharides and it will require a future research to understand its molecular mechanism of action.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".